Cell Fusion/Hybridoma Production ProtocolExplore further
2008-9-5u2002·u2002150ul medium/well Use 30ul to pick clones 3. When 96-well clones are just visible by eye (usually 3 days later), test by ELISA. Test supernatants for IgG (or other fusion protein tag) and the protein of interest in parallel. 4. For clones that recognize the protein of interest and not the IgG or fusion protein tag, transfer to 24 well plates ...
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